Material: Ceramic bird feeders are made from clay that is kiln-fired to create a durable and weather-resistant container. Ceramic is an excellent choice for bird feeders because it is sturdy, easy to clean, and provides insulation to keep bird food dry and fresh. Design: Ceramic bird feeders come in a variety of designs, ranging from simple and traditional to elaborate and decorative. They may feature whimsical shapes like animals, flowers, or geometric patterns, adding charm and personality to your garden. Some feeders are hand-painted with vibrant colors and intricate details to enhance their visual appeal. Construction: The construction of a ceramic bird feeder typically consists of a main feeding chamber or tray where birdseed is placed, surrounded by perches or platforms for birds to land and feed. The feeder may have multiple feeding ports or openings to accommodate several birds at once. Size: Ceramic bird feeders come in different sizes to accommodate various bird species and feeding preferences. Some feeders are compact and designed for small birds like finches or chickadees, while others are larger and can accommodate larger birds like cardinals or blue jays. Hanging or Mounting: Ceramic bird feeders can be hung from trees, hooks, or bird feeder poles using built-in loops or chains. Some feeders come with mounting brackets or stakes for secure installation in the ground or on a flat surface like a deck or patio. Easy to Clean: Ceramic bird feeders are easy to clean and maintain, requiring only occasional rinsing with water and mild soap to remove dirt, debris, and bird droppings. It's essential to clean the feeder regularly to prevent the buildup of mold or bacteria that can harm birds. Weather Resistance: Ceramic bird feeders are generally weather-resistant and can withstand exposure to sunlight, rain, and freezing temperatures without cracking or fading. However, it's a good idea to bring the feeder indoors during extreme weather conditions to prolong its lifespan. Attractiveness to Birds: The design and placement of a ceramic bird feeder can influence its attractiveness to birds. Placing the feeder in a quiet, sheltered area with nearby vegetation for cover can help attract a variety of bird species. Additionally, using high-quality birdseed or offering a variety of food options can entice birds to visit the feeder regularly. Overall, a ceramic bird feeder is a charming and practical addition to any garden or outdoor space, providing a welcoming environment for birds while adding beauty and interest to your landscape. Bird Feeder,Glazed Ceramic Bird Feeder, Pottery Bird feeder,Ceramic Bird Feeder Yixing Bocai Pottery Co.,Ltd , https://www.bocaipottery.com
**Human ICTP ELISA Kit – For the Quantitative In Vitro Determination of Human Carboxy-terminal Telopeptide Type I (ICTP) in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Biological Fluids**
**For Laboratory Research Use Only. Not Intended for Diagnostic Procedures.**
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### **INTENDED USE AND TEST PRINCIPLE**
This Human ICTP ELISA Kit is designed for research purposes only and is not intended for use in diagnostic or therapeutic procedures. The kit utilizes a competitive enzyme-linked immunosorbent assay (ELISA) to quantitatively measure ICTP levels in biological samples.
The reaction involves a colorimetric detection system where the addition of a stop solution changes the color from blue to yellow. The intensity of the color is measured at 450 nm using a microplate reader. A standard curve is generated using serial dilutions of ICTP standards, allowing the concentration of ICTP in unknown samples to be determined by comparing their optical density (OD) values to the standard curve.
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### **SAMPLE COLLECTION AND STORAGE**
- **Serum**: Collect using a serum separator tube. Allow samples to clot for 2 hours at room temperature or overnight at 4°C before centrifugation at 2000×g for 20 minutes. Remove serum and analyze immediately or aliquot and store at -20°C. Avoid repeated freeze-thaw cycles.
- **Plasma**: Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g, 2–8°C. Store at -20°C. Avoid repeated freeze-thaw.
- **Cell Culture Supernatants, Tissue Homogenates, and Other Fluids**: Centrifuge to remove particulates. Analyze immediately or store at -20°C. Avoid repeated freeze-thaw.
- **Note**: Ensure proper centrifugation, and avoid hemolysis or contamination with cellular debris.
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### **MATERIALS REQUIRED BUT NOT SUPPLIED**
1. Incubator set at 37°C
2. Microplate reader capable of measuring absorbance at 450 nm
3. Precision pipettes, disposable tips, and absorbent paper
4. Distilled or deionized water
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### **REAGENTS PROVIDED**
All reagents should be stored at 2–8°C. Check expiration date on label.
| Component | 96 Determinations | 48 Determinations |
|----------|-------------------|-------------------|
| MicroELISA Strip Plate | 12×8 strips | 12×4 strips |
| Standard (6 vials) | 0.5 ml/vial | 0.5 ml/vial |
| Sample Diluent | 6.0 ml | 3.0 ml |
| HRP-Conjugate Reagent | 10.0 ml | 5.0 ml |
| 20X Wash Solution | 25 ml | 15 ml |
| Chromogen Solution A | 6.0 ml | 3.0 ml |
| Chromogen Solution B | 6.0 ml | 3.0 ml |
| Stop Solution | 6.0 ml | 3.0 ml |
| Closure Plate Membrane | 2 | 2 |
| User Manual | 1 | 1 |
| Sealed Bags | 1 | 1 |
**Note**:
- Standard concentrations: 800, 400, 200, 100, 50, 25 pg/mL
- If sample values exceed the highest standard, dilute with sample diluent.
- Use only manufacturer-supplied reagents. Do not mix reagents from different kits.
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### **INSTRUCTIONS FOR USE**
1. Bring all reagents to room temperature (20–25°C).
2. Prepare 1X Wash Solution by diluting 1 volume of 20X Wash Solution with 19 volumes of distilled/deionized water. Store at 2–8°C for 1 month.
3. Add 50 µL of standard or sample to each well (duplicate). Do not add anything to the blank well.
4. Add 100 µL of HRP-Conjugate Reagent to all wells except the blank. Cover with adhesive strip and incubate for 60 minutes at 37°C.
5. Wash the plate 4 times manually or automatically.
6. Add 50 µL of Chromogen A and 50 µL of Chromogen B to each well. Incubate for 15 minutes at 37°C, protecting from light.
7. Add 50 µL of Stop Solution to each well. The color should turn yellow. If green or uneven, gently tap the plate.
8. Read OD at 450 nm.
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### **DATA ANALYSIS**
- Plot average OD values (450 nm) of standards against their concentrations.
- Subtract blank OD from all readings.
- Determine sample ICTP concentration by interpolating OD values on the standard curve.
- Intra-assay CV <15%, Inter-assay CV <15%.
- Assay range: 25 pg/mL – 800 pg/mL
- Sensitivity: <1.0 pg/mL
- Cross-reactivity: No significant cross-reaction with other proteins.
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### **STORAGE AND SAFETY**
- Store unused strips at 2–8°C with desiccant.
- Long-term storage: -20°C for up to 6 months.
- Handle all biological samples with care. Wear gloves and dispose of waste properly.
- Substrate solutions must be handled carefully; avoid exposure to heat or flame.
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**Important Note:** This kit is for research use only. It is not validated for clinical diagnosis. Always follow safety protocols and handle all reagents with caution.