Aseptic operation-Tips for slanting inoculation

1. The nickel wire part of the front end of the inoculation ring is sterilized with an alcohol lamp red, and the back end iron bar is overheated 2. The front end of the test tube is overheated 3. Open the test tube cap 4. The test tube is tilted and placed in the inoculation ring 5. The front end of the test tube is overheated and the test tube is covered Cap 6. Sterile operation of inoculation ring burning red sterilization – Tips for plate inoculation

Take a single colony from the Plate (Method 1: The lid is slightly opened to cover the culture medium to avoid the bacteria from falling into the air) Method 2: The plate is picked up on the reverse side) Aseptic operation-Tips for sucking the ball

1. Squeeze out the air in the safety suction ball, and insert the sterilized glass pipette 2. The upward is for suction, the downward is for release

Aseptic operation-Tips for pipetting

1. Adjust the Pipetman scale (P1000 suction range 200 ~ 1000μl)

2. Insert the sterilized Tip (press firmly) 3. Press the button to the first stage (do not press to the bottom) 4. Go deep into the liquid surface 2 ~ 4 mm 5. Slowly release the button to absorb the liquid Sterile operation-inoculation tips

1. The front end of the test tube is overheated 2. Open the test tube cover for aseptic operation-liquid inoculation technique

Method one: Inoculate the ring-contaminated bacteria and put them in a new medium for inoculation

Method 2: Use a safe suction ball to absorb the bacterial solution, put it into a new medium, and drain the bacterial solution downward

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