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Speaking of image processing software for scientific laboratories and microscope utilization, it is generally focused on the performance of image analysis software packages. However, in the actual selection and use of software, it is not only the problem in this aspect.
A new trend is the use of microscopy in image processing: the use of Adobe Photoshop is an assistant or substitute for conventional image processing.
In the United States, a technology marketing company that serves the field of microscopy and related imaging has passed several years of research in major industries such as the American Society for Cell Biology and the M&M Society (Microscopy Research Institute and Microbeam Analysis Society). This trend was followed for several years.
Nearly 90% use of Photoshop
The purpose of this article is certainly not to preview a particular software program. Photoshop's rapid and widespread spread was unexpected, but it was not noticeable at all.
With the development of science and technology, the situation in the academic world and advanced national laboratories is that about 80% of professional papers use Photoshop, and 90% of graduates also use this software.
The M&M figures for this year show that the use of laboratory managers (90%) is up from 84% in M&M in 1999 and 80% in 1998 in cell biology, and the use of laboratory staff has also been significant in the past year. The growth - reached 80%.
Industrial equipment also showed significant growth this year, from 63% to 75% in 1999. The data from clinical laboratories in the study are not sufficient to form statistically valid opinions. The law-based analysis at the M&M conference showed that the biologists and material scientists had slightly different opinions. Biologists believe that 90% of the use rate, and material scientists believe that in 1999 and 2000 were at 77%.
What are they doing?
Although Photoshop is designed for visual arts and general digital photography, some of its major functions are also well-applied in science.
For example, the control is a difficult point in microscopy. We usually have to drag hidden information to hidden or highlighted areas. Photoshop is a good tool for making comparisons. If the draft includes image analysis parts, measurements must be taken prior to processing with Photoshop to maintain the validity of scientific content.
In addition, annotations are important because many microscopic images are used for presentations, or for posters in publishing or professional conferences. As mentioned below, Photoshop's lamination function allows additional text, arrows, and scales to be placed in a separate layer and can be independently modified without affecting the image.
The third key feature is the scaling of the image. Most publishers have their special length and width requirements and resolution of 150 lines per inch (equivalent to 300 dots per pixel or resolution per inch). Photoshop can be easily scaled and adjusted. Using Photoshop to modify the image quality and file size is a very interesting process. It is important that if you change the resolution of the image, click the "resampling" button to keep the size of the image.
Photoshop also has a "batch" function to process groups of images, which saves a lot of time for image compression. Some people say that TIFF is the only image format officially approved by the American Microsurgery Society. For TIFF images, Photoshop offers the LZW compression feature for selection, which preserves image integrity through non-invasive calculations. In addition, multiple images can be mixed into a larger image.
Finally, for those looking for simple and inexpensive image analysis software, using Photoshop with plug-ins is also a good choice.
Lamination: A Secret to Photoshop's Success Long-term practice shows that Photoshop's two strengths are lamination and color balance.
Lamination is the division of an image into discontinuous image planes for individual processing. As mentioned earlier, this is a good method for annotations and image scales. Moreover, stereo photos can be independently colored (red/green, red/blue, etc.) without layering.
Secret: Design-to-Design Color Balance Knowing how to balance the color output from the camera to the monitor to the printer is a key issue. Since digital imaging involves many factors, it is indeed difficult to accurately reproduce the images seen under the microscope. Photoshop's color management features the use of displays, terminals (web pages, presentations, prepress), color systems (RGB and CMYK), image modes (shallow microscopes will select full color, and SEM, laser scanning confocal and AFM use The user will select the two-color screen or color. Even the brand and model of the printer are taken into account. Our suggestion is: Start with a well-behaved example and then work with more subtle and harder samples.
ADOBE's achievements in the academic world have added more networking features to the Photoshop 6.0 release, including the ability to convert text to PDF, and Adobe ReadyImage functionality that can generate images for the web.
In addition, the attractive price ensures that the software is quickly and widely popular.
The arguably arguing party believes that Photoshop can handle 16-bit black and white and color images, supports various types of file formats, and facilitates the conversion of troublesome MAC images to PCs. And, once it is familiar with its main features, its menus are clear and simple. In fact, Photoshop offers more performance than ever before.
The opposite side believes that some people may feel that this is a good and a bad thing. Since it is easy to process images, although better quality images will be obtained, it may also lead to serious loss of scientific validity. In addition, the entire program is not intuitive and still requires in-depth research, although successive versions of this software have already paid considerable attention to this part. The MME-like course is a solution that provides a program that combines the main features of Photoshop with scientific imaging concepts, minimizing any drawbacks in practice.
A View of Scientific Ethics In any image processing, using Photoshop adds some commentary issues. Traditional science stipulates that any modification of the image should be based on the discussion of the method. And, always save the original image as a TIFF file. (A recent opinion that was inadvertently heard was: save the original image as a file, and use Photoshop to save another file). Some people think that the measurement should be before processing because the processing may have influence on the measured parameters.
Finally, there is a reminder of image processing: its internal scientific information may be changed or misrepresented. Faced with a lot of experimental methods, even a small prediction, it will take a long time to test
Adobe Photoshop : The Surprise Scientific Image Processing Software of Choice ? Photoshop uses in medical research