Fluorescence detection and principle



Fundamental:
The "excitation light" is emitted by the light source, reaches the sample cuvette container through fiber optic conduction or direct irradiation, the sample is excited by external energy, emits "emission light", and then passes through the fiber optic conduction or directly illuminates the "spectrometer" for detection. The spectrometer reads the corresponding data and completes the test.

The excitation light and emission spectrum mostly cover the ultraviolet to near infrared range. It is recommended to use an ultraviolet-visible-near infrared spectrometer, so that both the "excitation light" and the "emission light" can be detected.

Main components: spectrometer, light source, reflection probe, computer

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