Since the invention of the first optical microscope by Levin Hook, the microscope has been continuously updated to meet the needs of the observer. At present, the most commonly used biological cell culture is the fluorescence microscope, which can be used to observe the three fluorescences of green, red and blue. In addition, from the perspective of observing live cells and fixed cells, it can be divided into two types: inverted microscope and upright microscope. Let me talk about the fluorescence microscope of Olympus. The model of the machine I use is OLYMPUSBX51. The reason why many people choose Olympus fluorescence microscopes is largely due to the quite user-friendly design of their home software. This software is very powerful, from image shooting to image post-processing in one step, which can meet a variety of shooting details you can think of and unexpected. In terms of image acquisition, the software's live image acquisition pixels are relatively high, and the clarity is also very good. In addition, it is equipped with an image lock button, which is convenient for locking the observation area and convenient for the collection of multiple photos. When preparing for shooting, we must first make a good white balance of the photo. This is very important for any shooting. Olympus' fluorescence microscope has designed two white balance adjustment modes. In the white light mode, with a square focus frame of about 1cm as the center, select one-touch peripheral (onetouch) or peripheral block selection mode. Easy to complete the setting of white balance. In fluorescence mode, the focus frame needs to be reduced to the fluorescence area before white balance correction can be performed. In addition, the lens of his house is also quite clear, and is also equipped with a live picture shooting mode, which supports animation shooting for a period of time. As long as the shooting time range is set and a culture box is equipped, dynamic shooting of live cells can be achieved. In terms of image processing, the function is relatively complete, you can add a ruler, you can also achieve the overlapping effect of multi-color pictures, and the interface is very friendly, photographers who are not familiar with Photoshop, if you have this software, you will find it convenient a lot of. It is said that Olympus ’fluorescence microscope software has sold very well in the past few years, but in recent years it has been affected by piracy and sales have fallen rapidly. After all, this tens of thousands of software, in addition to its powerful function, is also quite expensive. Many companies that sell Olympus fluorescence microscopes also often install pirated software to customers in order to reduce costs. Software sales. Having said so many advantages, what are the shortcomings of Olympus' fluorescence microscope? I think it is the problem of the clarity of images of more than 40 times. Because of the objective lens of more than 20 times, the clarity of observation will be affected. Although his family also provides lens switching mode, but the effect improvement is not obvious. But if you do n’t mind trying the oil lens, Olympus ’fluorescence microscope provides a 100x objective lens. After dripping oil, the shooting effect can satisfy everyone, even the small organelles in the cell and the fluorescence generated by transfection. Sometimes the small particles can be observed very clearly, which can provide good evidence support for the publication of the article.
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