Roll Top Fence has triangular bending at the top and bottom, named as triangle bending fence and BRC fence, widely used in Southeast Asia and Europe. Roll Top Fence, BRC Fencing, Triangle Bending fence, P Type fence Anping County Hua Guang Wire Mesh Production Co.,Ltd , https://www.aphgwirefence.com
1). Filter the mobile phase and select different filters according to your needs.
2). Ultrasonic degas the mobile phase after suction filtration for 10-20 minutes.
3). Turn on the HPLC workstation (including computer software and chromatograph), connect the mobile phase pipeline, and connect the detection system.
4). Enter the main menu of the HPLC control interface, click manual to enter the manual menu.
5). It was useless for a while, or changed to a new mobile phase, you need to flush the pump and injection valve first. Rinse the pump directly at the water outlet of the pump with a needle. To flush the injection valve, under the manual menu, first click purge, then click start, and the speed should not exceed 10 ml / min when flushing.
6). To adjust the flow rate and use a new mobile phase for the first time, you can try the pressure first. The greater the flow rate, the greater the pressure, generally not more than 2000. Click injure, select the appropriate flow rate, click on, walk the baseline, and observe the situation of the baseline.
7). Design aliasing methods. Click file, select select users and methods, you can select the existing various aliasing methods. To create a new method, click new method. Select the required accessories, including injection valve, pump, detector, etc., depending on the needs. After selecting, click protocol. A complete sampling method needs to include: a. Steady flow before injection, generally 2-5 minutes; b. Baseline zero; c. Loading-inject conversion of the injection valve; d. Sampling time, which varies with different samples different.
8). Inject and operate after injection. Select the aliasing method and click start. For injection, all samples need to be filtered. After the method is finished, click postrun to record data and mark. After all the samples have been walked, use the above method to walk the baseline for a while to wash off the residue.
9). When shutting down, turn off the computer first, then turn off the liquid chromatography.
10). Fill in the register and sign it by the person in charge.
Precautions:
1). The mobile phase requires chromatographic purity, and 20M deionized water is used for water. The mobile phase after degassing should be vibrated carefully so as not to cause bubbles.
2). The column is very fragile, the first time you do it, don't let liquid pass through the column.
3). All liquid passing through the column must be strictly filtered.
4). The pressure cannot be too great, it is best not to exceed 2000 psi
Operation steps of high performance liquid chromatograph:
1). Filter the mobile phase and select different filters according to your needs.
2). Ultrasonic degas the mobile phase after suction filtration for 10-20 minutes.
3). Turn on the HPLC workstation (including computer software and chromatograph), connect the mobile phase pipeline, and connect the detection system.
4). Enter the main menu of the HPLC control interface, click manual to enter the manual menu.
5). It was useless for a while, or changed to a new mobile phase, you need to flush the pump and injection valve first. Rinse the pump directly at the water outlet of the pump with a needle. To flush the injection valve, under the manual menu, first click purge, then click start, and the speed should not exceed 10 ml / min when flushing.
6). To adjust the flow rate and use a new mobile phase for the first time, you can try the pressure first. The greater the flow rate, the greater the pressure, generally not more than 2000. Click injure, select the appropriate flow rate, click on, walk the baseline, and observe the situation of the baseline.
7). Design aliasing methods. Click file, select select users and methods, you can select the existing various aliasing methods. To create a new method, click new method. Select the required accessories, including injection valve, pump, detector, etc., depending on the needs. After selecting, click protocol. A complete sampling method needs to include: a. Steady flow before injection, generally 2-5 minutes; b. Baseline zero; c. Loading-inject conversion of the injection valve; d. Sampling time, which varies with different samples different.
8). Inject and operate after injection. Select the aliasing method and click start. For injection, all samples need to be filtered. After the method is finished, click postrun to record data and mark. After all the samples have been walked, use the above method to walk the baseline for a while to wash off the residue.
9). When shutting down, turn off the computer first, then turn off the liquid chromatography.
10). Fill in the register and sign it by the person in charge.
Precautions:
1). The mobile phase requires chromatographic purity, and 20M deionized water is used for water. The mobile phase after degassing should be vibrated carefully so as not to cause bubbles.
2). The column is very fragile, the first time you do it, don't let liquid pass through the column.
3). All liquid passing through the column must be strictly filtered.
4). The pressure cannot be too great, it is best not to exceed 2000 psi
Operation steps of high performance liquid chromatograph: