Solid Phase Microextraction (SPME) Operating Procedure SOP Solid Phase Micro-Extraction (SPME): A solvent-free, fast, easy-to-extract, on-site sampling instrument. Can be applied in a wide range of fields; such as pharmaceutical analysis, food Analysis, environmental pollution analysis (VOC, PAH, PCB, organochlorine, organophosphorus pesticides) and so on. First, the installation program (A) Loosen the black protective sleeve under the Holder and loosen the stainless steel cover. Note: The stainless steel metal cover sometimes looses the Holder and is stuck in the black protective sleeve. In this case, please put the black protective sleeve back into the Holder and tighten it, then loosen the stainless steel. The cover will return to the outside of Holder's teeth, then remove it and leave Holder. (B) Push the Holder pusher to the lower end, where the black round bar is bald (with teeth inside). (C) Take out the Fiber to be used, and lock the upper teeth of the Fiber and the black stalk of the Holder's black bald rod. After the lock is smoothly closed, the push rod is carefully pulled back to the top, and then The stainless steel metal cover and the black protective sleeve that have just been removed are carefully locked and locked, that is, the device is completed. (D) Carefully try to push the Holder pusher to the middle end of the card. At this point, the Coated Fiber has been bald from the protective needle and the black protective cover can be adjusted up and down. Tube and view the Holder upper scale, slightly estimating the length of the protective needle plus Coated Fiber to match the experimental needs. Note: After the Fiber is installed, do not push the push rod to the lower end at this time, it will crush the Fiber spring. (E) When the SPME is to be inserted into the sample vial (bag) for sampling or inserted into the separator of the GC syringe for thermal desorption, first insert the protective needle into the spacer, then push the push rod Stuck to the middle end, bald out of Coated Fiber. (F) When the SPME is sampled from the vial (bag) barrier or removed from the GC syringe septum, the Coated Fiber is first retracted to the protective needle and the protective needle is removed. Note: Since the protective needle is a flat needle, the new spacer is first inserted with a clean fine needle to protect the needle from insertion. GC Injection Liner (Sleeve), please use instead SPME uses Liner like this Coated Fiber will not break. (G) To remove the Fiber from Holder, first pull the Holder pusher back to the top, let the Coated Fiber retract inside the protective needle, and place the black protective cover under the Holder. The tube is loosened and the stainless steel metal cover is removed, and then the Holder push rod is pushed to the lower end, so that the black round bar is bald, and the fiber can be loosened by the upper arm. The main part of the solid phase micro-extraction device is a handle with a solid phase micro-extraction head for operation, which is the simplest configuration. It is also best to have a constant temperature, magnetic stirring device Wait. You can also use the fully automatic SPME sampler, which is convenient and easy to use, which is too expensive. Second, the composition The SPME consists of a handle (Holder) and an extraction head (Fiber). Strong like a hue syringe, the extraction head is a fused silica fiber coated with different chromatographic stationary phases or adsorbents, stainless steel wire, thin stainless steel needle tube (protected quartz) The fiber is not broken and injected. The fiber head can be telescoped in the needle tube, and the handle is used to mount the extraction head for permanent use. Third, SPME operation steps Sample extraction 1. Insert the SPME needle through the vial septum and insert it into the bottle. 2. Push the handlebar to extend the fiber tip out of the needle tube. The fiber tip can be immersed in an aqueous solution (immersion) or placed in the upper space of the sample (headspace) for an extraction time of approximately 2-30 minutes. 3. Retract the fiber head and then withdraw the needle from the vial GC analysis 1. Insert the SPME needle into the GC inlet. 2. Push the handlebar, extend the fiber tip, and thermally desorb the sample into the column. 3. Retract the fiber head and remove the needle. principle: Solid Phase Micro-Extraction (SPME) is coated with a surface coating with a specific coating such as polydimethylsiloxane. Quartz fiber to adsorb volatile compounds in the headspace of plants or insects. Different coatings can be selected depending on the nature of the volatiles to be extracted. During the extraction process, the quartz fiber is The handle pushes out the protective needle sleeve, which is in the selected material, the volatile matter is adsorbed onto the fiber coating, and then the quartz fiber is directly injected into the inlet of the gas chromatograph and adsorbed on the surface layer. The volatiles are rapidly desorbed under transient high temperature conditions and separated into the column. SPME can continuously sample living organisms in natural or semi-natural conditions. The most accurate method for identifying small amounts of volatile compounds. The biggest advantage of this method is that it requires only a small amount of sample material and does not require an intermediate step of purification, so there is no solvent interference. with This method can basically reflect the types and concentrations of information compounds used by insects in the field to find food. The disadvantage is that the amount of information collected is small and can only be used for chemical analysis. For use in bioassays. Pet Nest Pet Nest DongGuan Lucky Pet Products Co., Ltd. , https://www.dgluckypet.com