The animal ELISA kit uses a specific reaction of an antigen and an antibody to connect the analyte to the enzyme, and then generates a color reaction by the enzyme and the substrate for quantitative determination. The subject to be measured may be an antibody or an antigen. The main functions are:

1. The amplification of the enzyme-catalyzed reaction is combined with the high specificity and specificity of the antigen-antibody affinity reaction, and the enzyme-labeled antigen or antibody is used as a main reagent for immunoassay, so that it has high sensitivity.

2. Realize the location of the trace antigen or antibody at the cellular or subcellular level, or quantify it at micrograms or even nanograms.

3. Its antigen-antibody reaction is highly specific.

4. It is a concentrate with a diluent, which can be directly used after dilution. No additional configuration is required.

Method classification:

Double antibody sandwich method

Indirect method

3. Competition law

4. Two-site one-step method

5. Capture assay for IgM antibodies

6. ELISA using avidin and biotin

When measured, the antigen (antibody) first binds to the solid support, but retains its immunological activity, and then adds an antibody (antigen) to the enzyme to form a conjugate (label), the conjugate still retains its The original immunological activity and the enzymatic activity, when the conjugate reacts with the antigen (antibody) on the solid phase carrier, and then add the corresponding substrate of the enzyme, that is, the catalytic hydrolysis or redox reaction to form a color.

The color depth produced is proportional to the amount of antigen (antibody) to be measured. Such colored products can be observed by naked eyes, optical microscopy, electron microscopy, or by spectrophotometer (microplate reader). The method is simple, convenient for speed and specific.

Vacuum bag

Vacuum bag,High Quality Vacuum bag,Vacuum bag Details, CN

Dongguan Yoteng Technology Co., Ltd. , https://www.ytpropackage.com